Invitrogen™ MICROBEnrich™ Kit

Invitrogen™ MICROBEnrich™ Kit

For the separation and isolation of bacterial RNA from mixed samples. The Ambion® kit includes sufficient reagents for depleting 500 µg of host cell RNA (20 reactions x 25 µg RNA) from a complex host-bacterial RNA mixture. Gene expression analysis following host-bacterial interactions is revolutionizing our understanding of the host response to bacterial infection. Numerous studies have utilized microarray analysis to follow host cell transcriptome alterations in response to interactions with bacteria. However, because no method has existed to isolate bacterial RNA away from host cell RNA, the corresponding analyses of bacterial transcriptomes has been impossible. The MICROBEnrich™ Kit enables whole genome microarray expression analyses of bacterial RNA after host-bacterial interactions.

Features

• Start With Any Total RNA Sample
• Remove >90% of Mammalian RNA from Complex Total RNA Mixtures
• The MICROBEnrich™ Kit Will Work With Any Bacterial Species

Contents

• Control RNA (1 mg/mL): 27 μL
• Capture Oligo Mix: 210 μL
• Glycogen (5 mg/mL): 100 μL
• 3 M NaOAc (sodium acetate): 1 mL
• Oligo MagBeads : 1.375 mL
• Binding Buffer: 9 mL
• Wash Solution: 2.4 mL
• Regeneration Solution 1: 2.8 mL
• Regeneration Solution 2: 5.5 mL
• Resuspension Solution: 1.375 mL
• Nuclease-free Water: 5 mL
• 1.5 mL tubes: 45

Collection Tubes (2 mL): 21