Invitrogen™ PureLink™ Expi Endotoxin-Free Giga Plasmid Purification Kit

Invitrogen™ PureLink™ Expi Endotoxin-Free Giga Plasmid Purification Kit

The PureLink Expi Endotoxin-Free Giga Plasmid Purification Kit enables quick and simple isolation of endotoxin-free plasmid DNA using an enhanced anion exchange membrane. In as little as 2 hours, purify up to 15 mg of high-quality plasmid DNA that is suitable for advanced transfection applications such as transfection of sensitive cells lines or genome engineering.

Features

• Simple and fast protocol—purification typically takes 2 hours
• High yield—isolate up to 15 mg of high quality plasmid DNA
• Endotoxin-free plasmid—<0.1 EU/µg, ideal for sensitive applications
• High purity—ultralow levels of protein, RNA, and genomic DNA contamination
• Compatibility—suitable for use with ExpiCHO and Expi293 expression systems
• Endotoxin-free components— elution buffer, water, and plasmid storage tubes that are free of endotoxin
  
  

Contents

• Resuspension Buffer (R3): 275 mL
• RNase A: 1.5 mL
• Lysis Indicator: 300 μL
• Lysis Buffer (L7): 275 mL
• Precipitation Buffer (N3): 275 mL
• Equilibration Buffer (EQ1): 440 mL
• Wash Buffer (W8): 3 × 440 mL
• Endotoxin Removal Buffer: (ER) 62.5 mL
• Elution Buffer (E4): 440 mL
• TE Buffer (TE): 30 mL
• Endotoxin Free Water: 21 mL
• Expi Gigaprep Lysate Filtration Cartridge: 2 each
• Expi Gigaprep DNA-Binding Cartridge: 2 each
• Pyrogen-free, 2-mL Tubes: 4 each