Invitrogen™ PureLink™ Expi Endotoxin-Free Maxi Plasmid Purification Kit, 25 Preps

Invitrogen™ PureLink™ Expi Endotoxin-Free Maxi Plasmid Purification Kit, 25 Preps

The PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit enables quick and simple isolation of endotoxin-free plasmid DNA using an enhanced anion exchange membrane. In as little as 90 minutes, purify up to 1.5 mg of high-quality plasmid DNA that is suitable for advanced transfection applications such as transfection of sensitive cells lines or genome engineering.

Features

• Simple and fast protocol—purification typically takes 90 minutes
• High yield—isolate up to 1.5 mg of high quality plasmid DNA
• Endotoxin-free plasmid—<0.1 EU/µg, ideal for sensitive applications
• High purity—ultralow levels of protein, RNA, and genomic DNA contamination
• Compatibility—suitable for use with ExpiCHO and Expi293 expression systems
• Endotoxin-free components— elution buffer, water, and plasmid storage tubes that are free of endotoxin
• Purification Target: Plasmid DNA
• Sample Type: Bacteria
• Purification Time: 90 min

Contents

• Resuspension Buffer (R3): 275 mL
• RNase A: 1.5 mL
• Lysis Indicator: 1 mL
• Lysis Buffer (L7): 275 mL
• Precipitation Buffer (N3): 275 mL
• Wash Buffer (W8): 2 × 510 mL
• Endotoxin Removal Buffer (ER): 62.5 mL
• Elution Buffer (E4): 440 mL
• TE Buffer (TE): 30 mL
• Endotoxin Free Water: 2 × 21 mL
• Lysate ClarificationColumn (in 50‑mL conical tube with white cap): 25 each
• Maxi DNA-binding Column: 25 each
• 50‑mL conical tube with blue cap: 25 each
• Endotoxin-free, 2-mLTubes: 25 each