Invitrogen™ PureLink™ Fast Low-Endotoxin Midi Plasmid Purification Kit, 50 Preps
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
50 Preps
Order number :
A36227
Invitrogen™ PureLink™ Fast Low-Endotoxin Midi Plasmid Purification Kit, 50 Preps
The PureLink Fast Low-Endotoxin Midi Plasmid Purification Kit uses next-generation columns with advanced silica membranes that enable isolation of transfection grade (<1 EU/µg) plasmid DNA in about 30 minutes. The simple precipitation-free protocol yields up to 0.4 mg plasmid, suitable for standard transfections and all molecular biology applications such as cloning and sequencing.
NOTE: The use of a vacuum manifold is required to perform this procedure. We recommend our FastVac Vacuum Manifold (A35899).
Features
- Ultra-fast protocol—innovative silica-based columns enable purification of transfection grade plasmid DNA in under 30 minutes
- Low endotoxin plasmid—the resulting plasmid contains <1 EU/µg endotoxins
- High yields—isolate up to 0.4 mg of high-quality plasmid DNA
- Colored buffers—the kit features colored buffers that permit error-free visualization of complete bacterial cell lysis and subsequent neutralization
Contents
- Resuspension buffer1 (red): 410 mL
- Lysis buffer[2] (blue): 410 mL
- Precipitation buffer (yellow): 410 mL
- Binding buffer: 410 mL
- Wash buffer 1: 55 mL
- Wash buffer 2 (concentrate): 23 mL
- Elution buffer: 12 mL
- Column assembly 3: 50 columns
- Syringe filters: 50 filters
- Syringe plungers: 50 plungers
- Collection tubes: 50 tubes
- Quick reference card: 1 card
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