Invitrogen™ PureLink™ HiPure BAC Buffer Kit

Invitrogen™ PureLink™ HiPure BAC Buffer Kit

The PureLink® HiPure BAC Buffer Kit provides additional buffers and reagents for performing BAC (Bacterial Artificial Chromosome) DNA isolation using the PureLink® HiPure Plasmid Kits. The PureLink® HiPure Plasmid Kits utilize a proprietary anion-exchange resin which allows you to purify high-quality BAC DNA from E. coli cultures. BAC DNA requires the use of large bacterial culture volumes to obtain a reasonable DNA yield. The additional buffers and reagents needed for working with larger bacterial cultures are provided in this Buffer Kit. From a 100 ml culture, you can expect to purify ∼40 µg of BAC DNA. The PureLink® HiPure BAC Buffer Kit provides reagents sufficient for five BAC Maxipreps, or 25 BAC Midipreps, or 100 BAC Minipreps.

Features

• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Purification Target: BAC DNA, Plasmid DNA
• Sample Type: Bacterial Culture

Contents

• Resuspension Buffer (R3): 250 mL
• Lysis Buffer (L7): 250 mL
• Precipitation Buffer (N3): 250 mL
• RNase A (20 mg/mL): 5 mL