Invitrogen™ PureLink™ HiPure Expi Plasmid Gigaprep Kit

Invitrogen™ PureLink™ HiPure Expi Plasmid Gigaprep Kit

The PureLink™ HiPure Expi Plasmid Gigaprep Kit enables isolation of the highest levels of transfection-grade plasmid DNA from a single E.coli giga prep using an enhanced anion exchange resin. The PureLink™ HiPure Plasmid Gigaprep Kit also includes vacuum filtration columns that provide bacterial filtration without centrifugation, enabling extremely fast process times. The PureLink™ HiPure Expi Plasmid Gigaprep Kit typically isolates 12 mg of high quality, ultrapure plasmid DNA with inherently low endotoxin levels from 2.5 L of bacterial culture.

Features

• High yield—isolate up to 14 mg of high quality plasmid DNA from a single purification using 5 L of bacterial culture volume
• Purity—inherently low endotoxin levels (typically 0.1–1.0 EU/µg) and A260/280 >1.8, making it ideal for mammalian cell transfection
• Simple and super-fast protocol—with vacuum assisted protocol, typical plasmid isolation in as little as 90 minutes

Contents

• Equilibration Buffer:  400 mL
• Resuspension Buffer:  250 mL
• Lysis Buffer:  250 mL
• Precipitation Buffer:  250 mL
• RNase A:  1.5 mL
• Wash Buffer:  3 × 400 mL
• Elution Buffer:  400 mL
• TE Buffer:  30 mL
• Expi HiPure Giga Filtration Column:  2 Each
• Expi HiPure Giga DNA Binding Column:  2 Each