Invitrogen™ PureLink™ HiPure Expi Plasmid Megaprep Kit

Invitrogen™ PureLink™ HiPure Expi Plasmid Megaprep Kit

The PureLink™ HiPure Expi Plasmid Megaprep Kit enables isolation of the highest levels of transfection-grade plasmid DNA from a single E.coli mega prep using an enhanced anion exchange resin. The PureLink™ HiPure Plasmid Megaprep Kit also includes vacuum filtration columns that provide bacterial filtration without centrifugation, enabling extremely fast process times. The PureLink™ HiPure Expi Plasmid Megaprep Kit typically isolates 4 mg of high quality, ultrapure plasmid DNA with inherently low endotoxin levels from 500 mL of bacterial culture.
PureLink™ HiPure Expi plasmid purification kits use a novel, proprietary anion-exchange resin to enable the purification of the highest levels of transfection-grade plasmid DNA. The enhanced resin combines large capacity with fast flow rate, high resolution, and efficient endotoxin removal. Plasmid preparations are typically completed in less than 2 hours with purity equal to two CsCl gradients. PureLink™ HiPure Expi plasmid purification kits enable the quickest process for obtaining the highest amount of transfection-grade plasmid DNA.

Features

• High yield—isolate over 5 mg of high quality plasmid DNA from a single purification using 1 L of bacterial culture volume
• Purity—inherently low endotoxin levels (typically 0.1–1.0 EU/µg) and A260/280 >1.8, making it ideal for mammalian cell transfection
• Simple and super-fast protocol—with vacuum assisted protocol, typical plasmid isolation takes as little as 90 minutes
• Purification Target: Plasmid DNA

Contents

• Equilibration Buffer: 400 mL
• Resuspension Buffer: 250 mL
• Lysis Buffer: 250 mL
• Precipitation Buffer: 200 mL
• RNase A: 1.5 mL
• Wash Buffer: 4 × 400 mL
• Elution Buffer: 400 mL
• TE Buffer: 30 mL
• Expi HiPure Mega Filtration Column: 4 Each
• Expi HiPure Mega DNA Binding Column: 4 Each