Invitrogen™ PureLink™ HiPure Plasmid Filter Midiprep Kit, 50 Preps

Invitrogen™ PureLink™ HiPure Plasmid Filter Midiprep Kit, 50 Preps

The PureLink® HiPure Plasmid Filter Midiprep Kit is designed to isolate plasmid DNA of the highest purity from 25 to 100 mL of an overnight bacterial culture. The PureLink® HiPure Plasmid Filter Kits include HiPure Filter Columns, which combine filtration for clarification of the bacterial lysate with anion-exchange resin purification of plasmid DNA, in a single unit. The resulting plasmid DNA is of a quality equivalent to that obtained by purification with two passes through cesium chloride gradients—the most rigorous method for DNA purification. Purify high-quality DNA in less than two hours for transfections and other applications with no need for additional steps to remove contaminants like RNA, proteins, or endotoxins. Additionally, the elimination of phenol, chloroform, ethidium bromide, and cesium chloride from the protocol minimizes exposure to, and disposal of hazardous materials.

Features

• Simple protocols—bacterial lysate clarification without centrifugation
• Fast results—save time by purifying plasmid DNA in less than two hours
• High yields—up to 200 µg for midipreps and up to 850 µg for maxipreps (see table)
• Reliable performance—the high-quality plasmid DNA purified with these kits is suitable for a wide variety of applications
• Elution Volume: 5 mL
• Plasmid: <40kb, Low Copy Plasmid, High Copy Plasmid, BAC
• Prep Scale: 100-200 µg (Medium-Scale) Plasmid DNA

Contents

• Equilibration Buffer (EQ1): 2 × 400 mL
• Resuspension Buffer (R3): 500 mL
• Lysis Buffer (L7): 500 mL
• Precipitation Buffer (N3): 500 mL
• RNase A: 2.8 mL
• Wash Buffer (W8): 3 × 500 mL
• Elution Buffer (E4): 250 mL
• TE Buffer (TE): 15 mL
• HiPure Filter Columns: 50
• Column Holders: 10