Invitrogen™ PureLink™ HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit, 10 Preps

Invitrogen™ PureLink™ HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit, 10 Preps

The PureLink™ HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit isolates plasmid DNA, from cell pellet to ultrapure DNA, in less than one hour. Using the HiPure Filter Columns and HiPure Precipitators supplied in the kit, you can purify plasmid DNA without the centrifugation steps required in traditional isolation protocols. The quality of the purified plasmid DNA is equivalent to that obtained by two passes through cesium chloride gradients - the most rigorous method for DNA purification - making it suitable for transfection and other applications requiring high-purity plasmid DNA.

Features

• Simple protocols - bacterial lysate clarification and isopropanol precipitation without centrifugation
• Fast results - from E. coli cell pellet to high-purity, transfection-grade plasmid DNA in about 60 minutes
• High yields - up to 850 µg from a high-copy plasmid
• Elution Volume: 15 mL
• Plasmid: <40kb, Low Copy Plasmid, High Copy Plasmid, BAC
• Prep Scale: > 200 µg (Large-Scale) Plasmid DNA

Contents

• Resuspension Buffer (R3): 100 mL
• RNase A: 650 μL
• Lysis Buffer (L7): 100 mL
• Precipitation Buffer (N3): 100 mL
• Equilibration Buffer (EQ1): 300 mL
• Wash Buffer (W8): 2 x 300 mL
• Elution Buffer (E4): 250 mL
• TE Buffer (TE): 15 mL
• HiPure Filter Columns: 10 Each
• Column Holders: 3 Each
• PureLink® HiPure Precipitator Module: 10 Each