Invitrogen™ PureLink™ Pro 96 Genomic DNA Purification Kit

Invitrogen™ PureLink™ Pro 96 Genomic DNA Purification Kit

The PureLink® Pro 96 Genomic DNA Purification Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from a wide variety of sample types in a high-throughput platform. This single kit allows genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, using a familiar silica semi-skirted 96-well plate format.
The PureLink® Pro 96 Genomic DNA Purification Kit can be used with many different sample types, each with its own optimized protocol outlined in the manual. Examples of sample sources and amounts that can be used with the PureLink® Pro Genomic DNA Purification Kit include mammalian cells and tissue, FFPE tissue, Oragene™-preserved saliva, mouse and rat tails, non-nucleated whole blood (human, mouse), nucleated whole blood (bird), blood spot on paper, bacterial cells, and yeast cells.
The PureLink® Pro 96 Genomic DNA Purification Kit outperforms competitor products in both DNA yield and purity for multiple sample types, including blood. You are also able to get greater yields of higher-purity gDNA (determined by A260/A280 measurements) no matter what your sample type is; from bacteria to tissues to blood to cells. The enhanced PureLink® Lysis and Wash Buffers help consistently deliver purified gDNA samples with absorbance reading ratios of ∼1.8 for both A260/A280 and A260/A230, indicating no contamination carryover of protein or guanidine

Features

• Kit flexibility—one kit works with a variety of sample types and sizes
• Ultraclean gDNA—excellent A260/A280 and A260/A230 absorbance ratios, regardless of sample source
• Improved design—optimized, semi-skirted, 96-well plates compatible with centrifugation and most benchtop and robotic vacuum manifolds
• Elution Volume Max: 150 µL
• Elution Volume Min: 50 µL (centrifuge), 100 µl (vacuum)
• High-throughput Compatibility: High-throughput Compatible, Not High-throughput Compatible (Manual)
• Prep Scale: 100 µl - 1 ml Blood Samples ( Medium-Scale)
• For Use With (Equipment): Biomek™ FX, Robotic Liquid Handling Systems, Vacuum Manifold, Eppendorf epMotion, Centrifuge, TECAN Freedom Evo, TECAN Genesis
• Purification Target: Genomic DNA
• Sample Type: Bacteria (Gram-Negative), Bacteria (Gram-Positive), Buccal Sample, Cell Cultures, Insect Cells, Mammalian Cells, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Tissue (Mammalian), Whole Blood, Yeast, Saliva, Fixed Cells

Contents

• PureLink™ Pro 96 Genomic Lysis/Binding Buffer: 80 mL 
• PureLink™ Pro 96 Genomic Digestion Buffer: 70 mL 
• PureLink™ Pro 96 Genomic Wash Buffer 1: 2 × 100 mL
• PureLink™ Pro 96 Genomic Wash Buffer 2: 2 × 75 mL 
• PureLink™ Pro 96 Genomic Elution Buffer: 160 mL 
• RNase A (20 mg/ml): 8 mL 
• PureLink™ Pro 96 Proteinase K (20 mg/ml): 8 mL 
• PureLink™ Genomic DNA Filter Plate: 4 
• 96 Deep Well Blocks: 2 × 6 
• PureLink™ Foil Tape: 20/pack