Invitrogen™ PureLink™ Pro 96 Viral RNA/DNA Purification Kit

Invitrogen™ PureLink™ Pro 96 Viral RNA/DNA Purification Kit

The PureLink® Pro 96 Viral RNA⁄DNA Purification Kit is the only 96-well silica plate system that can extract both viral RNA and DNA from 200-µl cell-free fluids which uses a low-depth 96-well centrifuge bucket for ease-of- use, all without any loss in sensitivity.
The PureLink® Pro 96 Viral RNA⁄DNA Purification Kit is based on conventional silica plate extraction chemistry. Cell-free samples are lysed in guanidine containing buffers that have been reformulated to increase the recovery and sensitivity of purified nucleic acids, while carrier RNA is used to protect samples from RNase degradation. Binding of lysates to the Viral Filter Plate can be performed with either a vacuum manifold or centrifugation using a 96-well rotor with buckets having a depth of only 5 cm. After washes with ethanol-based buffers, the viral RNA or DNA is eluted in 100 µl of RNase-free water. The RNA or DNA is ready to use in one-step or two-step RT-PCR and qRT-PCR, qPCR, or other enzymatic amplification procedures.
The PureLink® Pro 96 Viral RNA⁄DNA Purification Kit shows excellent well-to-well consistency
A critical requirement for any 96-well purification system is well-to-well consistency. When using a technique as sensitive as qRT-PCR for viral nucleic acid detection, which may exist as low viral titers from patient samples, consistency between samples becomes even more important. When well-to-well recovery of lentiviral RNA was analyzed using the PureLink® Pro 96 Viral RNA⁄DNA Purification Kit, consistent Ct values were obtained in qRT-PCR analyses while plasma controls run on the same plate were negative, showing no cross contamination

Features

• Versatility-one kit for all your samples, the protocol is optimized for both viral RNA and DNA extraction
• Flexibility in centrifuge choice-minimum speeds for 96 well processing of 2250 x g and a rotor bucket depth of 5 cm make this kit one of the most adaptable systems for existing laboratory centrifuges
• Sensitivity and reproducibility as good as a spin column-moving to high throughput is reliable and easy with no loss in accuracy of quantitation
• For Use With (Equipment): Biomek™ FX, Robotic Liquid Handling Systems, Vacuum Manifold, Eppendorf epMotion, Centrifuge, TECAN Freedom Evo, TECAN Genesis
• Sample Type: Plasma, Cell-Free Fluids, Cerebrospinal Fluid, Serum, Liquid Samples (e.g. Serum), Viral Samples

Contents

• PureLink™ Pro 96 Viral Lysis Buffer (L22): 100 mL
• PureLink™ Pro 96 Wash Buffer II (5X): 2 × 87.5 mL
• Proteinase K (20 mg/ml) in storage buffer (proprietary): 10 mL
• Carrier RNA (lyophilized): 2.2 mg
• PureLink™ Pro 96 RNase-free Water: 75 mL
• PureLink™ 96 Well Viral RNA/DNA Filter Plate: 4
• 96 Deep-Well Block: 2 × 6/bag
• Foil Tape: 3 × 4/bag