Invitrogen™ PureLink™ Pro Quick96 Plasmid Purification Kit, 24 x 96 Preps

Invitrogen™ PureLink™ Pro Quick96 Plasmid Purification Kit, 24 x 96 Preps

The PureLink® Pro Quick96 Plasmid Kit combines advanced silica plate extraction chemistry with an optimized 96-well plate design for manual or automated processing of plasmid DNA from E. coli in as little as 45 minutes.
The PureLink® Pro Quick96 Plasmid Kit allows rapid and reproducible purification of up to 20 µg of plasmid DNA from E. coli strains grown in up to 5 ml LB, 3 ml 2xYT, or 3 ml TB. In an automated platform using 1.5 ml E. coli culture grown overnight in a square-well block, typical yields are 5-15 µg plasmid DNA, with an average of 9.4 µg.
The resulting plasmid DNA is supercoiled with no detectable genomic DNA or RNA. The PureLink® Pro Quick96 Plasmid Kit provides higher yields of high-quality plasmid DNA for use in common downstream applications such as automated sequencing, PCR, restriction enzyme digestion, and cloning.

Features

• Obtain consistently high yields of high-purity plasmid DNA
• Experience ease of use and better performance due to improved plate design and protocol
• Increase processing flexibility with protocols for centrifuge, vacuum manifold, or automated platforms
• For Use With Equipment: Biomek™ FX, Robotic Liquid Handling Systems, Vacuum Manifold, Eppendorf epMotion, Centrifuge, TECAN Freedom Evo, TECAN Genesis
• Elution Volume: 50 µlL (centrifuge), 100 µL (vacuum), 150 µL
• Prep Scale: < 100 µg (Small-Scale) Plasmid DNA

Contents

• PureLink™ Pro 96 Resuspension Buffer: 2 × 600 mL
• PureLink™ Pro 96 Lysis Buffer: 2 × 600 mL
• PureLink™ Pro 96 Neutralization Buffer: 2 × 600 mL
• PureLink™ Pro 96 RNase A: 24 mL
• PureLink™ Pro 96 Elution Buffer: 600 mL
• PureLink™ 96 Well Plasmid Filter Plate: 24 each
• PureLink™ Pro 96 Plasmid Clarification Plate: 24 each
• PureLink™ Pro 96 Wash Buffer I: 1 L
• PureLink™ Pro 96 Wash Buffer II (5X) (in 1 L bottles): 2 × 600 mL
• PureLink™ Pro Elution Plate: 24 each
• PureLink™ Pro 96 Growth Block (Square-well): 24 each
• PureLink™ Air Porous Tape: 24 each
• Foil Tape: 24 each
• PureLink™ Pro Quick96 Wash Buffer I - Dilution Bottle (1 L bottle): 1 each
• PureLink™ Quick 96 Wash Buffer II - Dilution Bottle (1 L bottle): 1 each