Invitrogen™ QuantiGene™ Sample Processing Kit, cultured cells, 10 Plates

Invitrogen™ QuantiGene™ Sample Processing Kit, cultured cells, 10 Plates

This QuantiGene Sample Processing Kit for cultured cells contains reagents and instructions for the preparation of cell lysates for use in QuantiGene Singleplex and QuantiGene Plex assays for RNA targets and QuantiGene Plex DNA assays for DNA targets.

The protocols provided with the QuantiGene Sample Processing Kit for cultured cells use a combination of these factors to ensure complete lysis of the samples. In addition, the lysis mixture provides the proper ionic conditions for the overnight hybridization in the QuantiGene assays.
Key factors that influence the complete lysis of the cells include:

• The lysis mixture
• Proteinase K
• Vortexing or pipetting of the sample
• Storage of the lysates at -80°C
• Incubation at 50°C

Features

• Easy to follow instructions
• Preserves the integrity of the RNA/DNA without degradation
• Choose the method that matches your cultured cell sample

Contents

• Lysis Mixture:   75 mL
• Proteinase K (50 µg/µL):   750 µL