Invitrogen™ S.N.A.P.™ UV-Free Gel Purification Kit

Invitrogen™ S.N.A.P.™ UV-Free Gel Purification Kit

The S.N.A.P.™ UV-Free Gel Purification Kit uses non-toxic reagents to rapidly purify intact DNA fragments from agarose gels. The key to isolating undamaged DNA is the unique crystal violet reagent, which allows visualization of DNA (>=200 ng) in the gel under ambient light. This eliminates the need for ethidium bromide and exposure to UV light, which can nick and damage DNA. Nucleic acids up to 7 kb are efficiently purified from the gel using the convenient S.N.A.P.™ resin, provided ready to use in a spin column format. The resin eliminates organic extractions, which generate hazardous waste. In addition, there are no time-consuming ethanol precipitations.

The S.N.A.P.™ UV-Free Gel Purification Kit yields pure, intact DNA in an easy-to-perform, rapid procedure. Simply:

1. Prepare gel with crystal violet reagent and load samples in crystal violet loading buffer.
2. Visualize DNA band under ambient light and excise from agarose gel.
3. Add NaI solution (provided) and melt the agarose at 42-50°C.
4. Load sample onto the S.N.A.P.™ spin column.
5. Wash away contaminants.
6. Elute DNA in water.

The eluted DNA is highly pure, intact, and ready to use for cloning, sequencing, transformation, or transfection.

Features

• Sample Type: DNA Fragments in Agarose Gel Slices, Gel Samples
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• In Gel Dye: Crystal Violet

Contents

• S.N.A.P.ô Columns: 25
• S.N.A.P.ô Collection Vials: 25
• Sodium Iodide Solution: 11 mL
• Binding Buffer: 15 mL
• 4X Final Wash: 6 mL
• Crystal Violet: 2 x 0.5 mL
• 6X Crystal Violet Loading Dye: 2 X 0.25 mL
• TE Buffer, pH 8.0: 2 mL
• Sterile Water Deionized: 2 x 1 mL