Invitrogen™S.N.A.P.™ Plasmid DNA MidiPrep Kit

Invitrogen™S.N.A.P.™ Plasmid DNA MidiPrep Kit

The S.N.A.P.™ MidiPrep Kit is a medium-scale plasmid DNA purification kit designed for fast and easy purification of pure plasmid DNA from 10-100 ml of overnight culture.
The S.N.A.P.™ MidiPrep procedure takes just 40 minutes. You simply:

• Lyse cells and filter debris with the S.N.A.P.™ filtering column
• Apply flow-through to the S.N.A.P.™ DNA binding column
•  Wash the column
• Elute pure, supercoiled plasmid DNA in water or TE buffer

Plasmid DNA purified with the S.N.A.P.™ MidiPrep Kit is ready to use right off the column. No precipitation is necessary. Plasmid is eluted in TE or water at ∼200 ng/µl-perfect for subsequent applications including mammalian cell transfection.

Features

• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Prep Scale: 100-200 µg (Medium-Scale) Plasmid DNA
• Purification Target: Plasmid DNA
• Sample Type: Bacterial Culture

Contents

• Column A (Filtering):  20
• Column B (Binding):   20
• Resuspension Buffer:  80 mL
• RNaseA, lyophilized:  6 mg
• Lysis Buffer:  80 mL
• Precipitation Salt:  80 mL
• Binding Buffer:  2 x 120 mL
• Wash Buffer:  100 mL
• 4X Final Wash: 80 mL