Ion Torrent™ Ion AmpliSeq™ Comprehensive Cancer Panel
On demandIon Torrent™ Ion AmpliSeq™ Comprehensive Cancer Panel
The Ion AmpliSeq™ Comprehensive Cancer Panel provides highly multiplexed target selection of genes implicated in cancer research. Encompassing over 50% of the Wellcome Trust Sanger Institute Cancer Gene Census, this is the most comprehensive cancer gene panel available. With all-exon coverage of 409 genes, the Ion AmpliSeq™ Comprehensive Cancer Panel delivers fast, FFPE-compatible, target selection for a broad survey of key genes for semiconductor sequencing. he Ion AmpliSeq™ technology allows ultra-high multiplex PCR combined with targeted sequencing library construction in just 16 hours for over 400 genes. Utilizing only 10 ng of input DNA per primer pool for a total of 40 ng of input DNA, analysis of restricted samples like FFPE samples is possible. The Ion AmpliSeq™ Comprehensive Cancer Panel is optimized for library construction with the Ion AmpliSeq™ Library Kit 2.0. Libraries are then ready for template preparation on the Ion OneTouch™ System and sequencing on the Ion PGM™ Sequencer. The Ion AmpliSeq™ Comprehensive Cancer Panel is not compatible with Ion Ampliseq™ 2.0 Beta Kits.
Features
- Broad survey of 409 key genes in a simple PCR reaction, no additional capital equipment required
- Unmatched plexy of 16,000 primer pairs in only four pools with Ion AmpliSeq™ technology
- Low DNA input of only 40 ng DNA and short amplicons enable FFPE samples and needle biopsies
- Start comprehensive genomic studies quickly with pre-designed primer pools
- Simplify analysis and annotation of variants with Ion Reporter™ software
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