Ion Torrent™ Ion Library TaqMan™ Quantitation Kit

Ion Torrent™ Ion Library TaqMan™ Quantitation Kit 

The Ion Library TaqMan™ Quantitation Kit is an easy-to-use, flexible solution for the accurate quantitation of library input for the Ion Torrent semiconductor sequencing workflow. This comprehensive kit provides a TaqMan® qPCR mix and library standard to detect and quantify femtomolar amounts of Ion Fragment Libraries. With universal cycling conditions that allow both fast and standard cycling, the Ion Library TaqMan Quantitation kit supports most real-time platforms. This universal kit provides greater accuracy and specificity compared to non-probe-based methods, delivering simplicity and speed to the PGM™ System sequencing workflow. 

Features 

• Precision— The TaqMan® Assay provides greater specificity and accuracy in detecting properly adapted, amplifiable templates over non-probe-based quantitation methods. 
• Sensitivity— The protocol supports quantitation of femtomolar amounts of fragment libraries. 
• Simplicity— The validated, ready-to-use qPCR standard requires only a simple serial dilution to determine the optimal concentration of library to use for templated bead preparation. 
• Versatility— The highly robust qPCR mix accommodates a wide range of cycling conditions and reaction volumes, and combines highly sensitive detection with a broad quantification range. 
• Efficiency— qPCR-based methods use less material for quantification. 

Contents

• Ion Library qPCR Master Mix
• Ion Library TaqMan™ Quantitation Assay, 20X
• E. coli DH10B Control Library