Ion Torrent™ Oncomine™ BCR IGH SR Assay, DNA
On demandIon Torrent™ Oncomine™ BCR IGH SR Assay, DNA
The Ion Torrent Oncomine BCR IGH SR Assay, DNA, is a robust, targeted next-generation sequencing (NGS) assay designed for use in hematology-oncology and minimal residual disease (MRD) monitoring research. It can also be used to investigate immunotherapy biomarkers, as well as in infectious and autoimmune disease research. The assay kit includes a single pool of multiplex PCR primers, total dNTPs, and library reagents.
The Oncomine BCR IGH SR Assay, DNA, accurately identifies and measures clonal expansion of B lymphocytes using genomic DNA from bone marrow, whole blood, peripheral blood leukocytes (PBLs), peripheral blood mononuclear cells (PBMCs), and fresh-frozen and formalin-fixed paraffin-embedded (FFPE) research samples. Using Ion AmpliSeq technology, the assay identifies unique B-cell clones through targeting of the highly diverse complementarity-determining region 3 (CDR3) of the B-cell receptor (BCR) immunoglobulin heavy (IGH) chain from a DNA template. The nucleotide sequence of the IGH CDR3 region serves as a natural barcode to enable clone identification for MRD clinical research. The measurements of B-cell clonal expansion and diversity are useful for pharmacodynamics and vaccine biomarker research. IGH CDR3-region amino acid motifs may reveal signatures of B-cell responses to defined antigens for use as future markers of autoimmune or infectious disease.
Features
- High sensitivity and exceptionally low limit of detection for rare clone identification via dual-barcode indexing and high template capture efficiency of Ion AmpliSeq multiplex PCR
- Compatibility with extracted DNA from an array of sample types, including fresh-frozen and FFPE tissue, bone marrow, whole blood, PBLs, and PBMCs
- Assay primers designed to amplify all variable and joining gene alleles in gold-standard IMGT database
- Generation of large libraries with minimal input requirement, facilitating study of rare clones
- Detection of clones of interest at a frequency as low as 10-6
- Efficient workflow with 48-hour library-to-results time
- Flexible input requirements ranging from 50 ng to 2 µg
- Streamlined and user-friendly informatics solution with automated clonotyping, clonal lineage analysis, reporting of key repertoire features, and multi-sample analysis capability to generate MRD analysis for research samples
- Ultra-low substitution error rate minimizing artifacts arising from sequencing errors, enabling highly accurate assessment of clonal heterogeneity in malignant B cells of interest in research samples
Contents
- Oncomine BCR IGH-SR Assay, DNA
- Ion AmpliSeq Library Kit Plus
- dNTP Mix, 25 mM each
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