Ion Torrent™ Oncomine™ BCR Pan-Clonality Assay (IGH, IGK, IGL, KDE/Cint- FR3-J), DNA, 24

Ion Torrent™ Oncomine™ BCR Pan-Clonality Assay (IGH, IGK, IGL, KDE/Cint- FR3-J), DNA, 24

The Oncomine BCR Pan-Clonality Assay is a targeted next-generation sequencing (NGS) assay for detection of B-cell heavy and light chain diversity to measure clonal expansion/contraction with accuracy and for detection of rare clones with high sensitivity. Primers have been specifically designed to target the FR3-J region of the IGH, IGK, and IGL rearrangements, as well as KDE and Cint-containing rearrangements. Through the use of Ion AmpliSeq technology, sequencing of all three chains of the B-cell receptor occurs in a single reaction. When combined with Ion Reporter Software, data can be easily analyzed and reports created within a short period of time.

For use with equipment: GeneStudio

Features

• Sequencing of CDR3 region of IGH, IGK, and IGL chains, including kappa deletion element and C-intron
• Reduced secondary testing—Ion AmpliSeq technology enables single reaction to sequence all three chains
• Detect rare clones at LOD of 10-6
• Optimized Oncomine informatics pipeline for easy, all-inclusive analysis and publication-ready data plots

Contents

• Oncomine BCR Pan-Clonality Panel
• dNTP mix
• Ion AmpliSeq Library Plus Kit