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Ion Torrent™ Oncomine™ BRCA Research Assay, Manual Library Preparation

On demand
Brand origin : United States
Expiry time : On demand
Delivery time : 1 Week/s
Delivery cost : On demand
Unit size : 24 Reactions
Order number : A32840
(Ex-work price)
$ On demand

Ion Torrent™ Oncomine™ BRCA Research Assay, Manual Library Preparation

 

The Oncomine™ BRCA Research Assay consists of two pools of AmpliSeq™ oligonucleotide primers and associated reagents to generate amplicon libraries for next-generation sequencing (NGS) on Ion Torrent™ platforms. The assay is designed to provide sensitive and comprehensive sample amplification of all coding regions of the human BRCA1 and BRCA2 genes. This version of the assay is for manual library preparation.

 

Features

  • Validated for use on Ion 318 and Ion 530 chips Detects SNVs, InDels, and large exon/gene deletions/duplications
  • Compatible with as little as 10 ng input DNA from formalin-fixed, paraffin-embedded (FFPE) tissue or blood samples
  • Highly uniform coverage across all coding exons and splice sites for efficient sequencing and accurate analysis
  • Optimized primer design and amplification chemistry enable highly specific target enrichment
  • Validated detection of somatic variants to 5% and lower
  • Simple and fast workflow produces targeted libraries in 3.5 hours typically

 

Contents

  • Oncomine™ BRCA Pool 1 (blue cap)
  • Oncomine™ BRCA Pool 2 (red cap)
  • Ion AmpliSeq™ Library Kit Plus

 

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