Ion Torrent™ Oncomine™ Comprehensive Assay v3 GX

Ion Torrent™ Oncomine™ Comprehensive Assay v3 GX

The Oncomine Comprehensive Assay v3 GX is a targeted, next-generation sequencing (NGS) assay that enables the detection of relevant SNVs, CNVs, gene fusions, and indels from 161 unique genes to help inform drug discovery research and clinical trial research programs. The assay kit provides reagents for library construction and four pool panels—two DNA and two RNA—of multiplex PCR primers for preparation of amplicon libraries to perform 16 sequencing reactions on formalin-fixed paraffin-embedded (FFPE) tumor samples. Designed to go from hundreds down to a few relevant cancer drivers, this assay is part of a comprehensive workflow that enables NGS data analysis from as little as 10 ng of nucleic acid per pool. The assay is optimized for sequencing on the Ion Torrent Genexus Integrated Sequencer using the Ion Torrent Genexus GX5 Chip. The Genexus Integrated Sequencer performs library preparation, sequencing, analysis, and reporting in an automated workflow to deliver results in a single day. The multi-lane sequencing chip is designed to enable cost-effective processing of libraries.

Features

• Analysis of variants across 161 genes Detection of SNVs, CNVs, gene fusions, and indels
• Robust performance from as little as 10 ng per pool (40 ng total) isolated from FFPE samples, including fine needle biopsies
• Characterized with molecular standards and controls
• Content driven by the Oncomine Knowledgebase and experienced scientists that helps assure coverage of key targets aligned to published evidence

Contents

• Oncomine Comprehensive Assay v3 DNA GX
• Oncomine Comprehensive Assay v3 RNA GX
• Ion Torrent Genexus Strip 1
• Ion Torrent Genexus Strip 2-AS