Ion Torrent™ Oncomine™ Immune Response Research Assay

Ion Torrent™ Oncomine™ Immune Response Research Assay 

The Oncomine™ Immune Response Research Assay is a targeted gene expression assay designed for the Ion™ next-generation sequencing (NGS) platform. This pan-cancer gene expression assay is designed to interrogate the tumor microenvironment to enable mechanistic studies and identification of predictive biomarkers for immunotherapy in retrospective or prospective clinical trial research cohorts. The assay is optimized to measure the expression of genes involved in tumor-immune interactions, including the low-expressing genes involved in inflammatory signaling. 

This assay contains the reagents for manual library construction and a single pool of primers used to perform multiplex PCR for preparation of amplicon libraries from formalin-fixed paraffin-embedded (FFPE) samples. The assay enables quantitative evaluation of the expression of markers associated with different leukocyte subsets, antigen presentation, checkpoint pathways, and tumor progression. This 400-gene assay is supported with a user-friendly informatics workflow and demonstrates high sensitivity for low-expressing transcripts derived from FFPE samples. The Oncomine Immune Response Research Assay leverages Ion AmpliSeq™ technology to deliver results from as little as 10 ng of total RNA, enabling robust performance from challenging FFPE samples. 

Features 

• High repeatability and sensitive detection of gene expression across different types of solid tumors 
• Low sample input requirement (10 ng of total RNA material) 
• High sensitivity detection of low-expressing signaling molecules such as interleukins and interferon gamma 
• High sample multiplexing with flexibility to run 8 samples per single run 
• Efficient workflow that offers sample-to-results in less than 48 hours 
• Automated and seamlessly integrated analysis solution 
• Interpretation of immune surveillance and tumor progression mechanism with functional annotation of genes and pathways

Contents 

• Oncomine Immune Response Research Oligos
• Ion AmpliSeq Library Kit 2.0
• 1 tube Low TE Buffer