Ion Torrent™ Oncomine™ Tumor Mutation Load Assay, manual library preparation

Ion Torrent™ Oncomine™ Tumor Mutation Load Assay, manual library preparation

The Oncomine Tumor Mutation Load Assay is a targeted next-generation sequencing (NGS) assay that provides an assessment of tumor mutation load and mutation signatures in a simple workflow. The assay provides reagents for library construction from two pools of multiplex PCR primers for preparation of amplicon libraries from formalin-fixed paraffin-embedded (FFPE) tumor samples without the need for a matched normal sample, using 10 ng of DNA per primer pool. This assay is designed to facilitate successful selection and identification of samples most likely to derive responses in cancer immunotherapy research.

Features 

• Enables accurate quantitation of somatic mutations to assess tumor mutation load 
• Single tumor sample workflow reduces the need for a matched normal sample
• Efficient amplification from challenging samples with AmpliSeq technology 
• Streamlined analysis with feature-rich visualization
• Efficient multiplexing of up to eight samples per Ion 540 Chip on the Ion S5 Systems 

Contents 

• 1 Oncomine Tumor Mutation Load Panel, 24 reactions
• 1 Ion AmpliSeq Library Kit Plus, 24 reactions