MGIEasy RNA Directional Library Prep Set (16 RXN)

MGIEasy RNA Directional Library Prep Set (16 RXN）

MGIEasy RNA Directional Library Prep Set provides an efficient solution for generating libraries suitable for MGI high-throughput sequencing platforms from total RNA. It can be used to identify RNA stranded information that from which of the two DNA strands a given RNA transcript originates. Identifying strand origin increases the percentage of reads that align to the transcriptome and provides more accurate information for studies in regulation of gene expression and gene functional analysis. Maintaining strand orientation also allows identification of antisense expression and novel genes. 

Features

• Wide range of total RNA input, Compatible with a range of total RNA input (10 ng-1 ug) and exhibits excellent quality sequencing data and transcript annotation.
• Compatible with various sample types, Including low-quality RNA, FFPE RNA and plasma samples.
• Excellent coverage quality, Accurate and comprehensive mapping of transcripts.
• High uniformity, Enables high uniform from transcripts 5 'to 3' ends and superior 3’end coverage.
• Accurate detection of strand orientation, Identify transcript origin, detect antisense transcripts, enhances transcript annotation.
• Easy to Use, Simple protocol and short operation time, suitable for automated library preparation instrument.
• Flexibility, Various RNA enrichment methods and sequencing read lengths to meet the needs of different sample types.
• Recommended sequencing data per sample, 25 M raw reads（SE50）/ 8 Gb raw data（PE100/PE150).

Contents

• Library, MGIEasy RNA Directional Library Prep Kit V2.0 (16 RXN): 1000005270
• Adapters, MGIEasy DNA Adapters-16(Tubes) Kit V1.0（16 RXN, 16 barcodes): 1000005284
• Clean Beads, MGIEasy DNA Clean Beads: 1000005278
• Circularization, MGIEasy Circularization Module V2.0: 1000005260

Related product

• MGIEasy rRNA Depletion Kit（32 RXN): 1000005953