Sarstedt™ MiniPERM®, Nutrient Module, Medium Compartment, For MiniPERM® Production Module
On demandminiPERM®, Nutrient Module, Medium Compartment, For miniPERM® Production Module
The miniPERM® is an easy-to-handle bioreactor which was developed for the cultivation of eukaryotic cells (mammalian, insect and plant cells) in high density and therefore for biomass production and for the production of cell products. The sub-division of the bioreactor into production and nutrient modules and the rotating cultivation allow for the production of highly concentrated cell products in small volumes. Therefore, depending on the cell line, cell densities of more than 107 cells/ml and product concentrations of several mg/ml can be achieved. This means that the miniPERM® bioreactor is a cost-effective and time-saving alternative to using conventional roller bottles and fermentation systems.
The principle
The miniPERM® bioreactor is subdivided into a production module and a nutrient module (two-compartment system) by a dialysis membrane. The dialysis membrane has a cut off size of 12.5 kDa so that neither cells nor secreted cell products can diffuse into the nutrient module. At the same time, the exchange of nutrients and cell metabolites takes place via the dialysis membrane. The gas exchange is effected via a thin, gas-permeable silicone membrane on the outer side of the production module.
Features
- High cell densities
- High product concentrations
- Multiple harvests
- Easy to use
- Cultivation of hybridoma cells for obtaining antibodies.
- Cultivation of transfected cells for obtaining recombinant proteins or for virus production.
- Biomass production
- autoclavable up to 121 °C
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