Thermo Scientific™ CaptureSelect™ HSA Ligand Leakage ELISA, 1 Assay

Thermo Scientific™ CaptureSelect™ HSA Ligand Leakage ELISA, 1 Assay

The CaptureSelect™ HSA Ligand Leakage ELISA detects contamination of CaptureSelect™ HSA affinity ligand in HSA or HSA fusion products purified using the CaptureSelect™ Human Albumin affinity matrix. The HSA affinity ligand, a 13 kD recombinant protein, is coupled by multipoint attachment through stable amide bonds to give high chemical stability and low leakage. Even when covalently attached, small amounts of the affinity ligand can leach off of the chromatography support and co-elute with the protein. This ELISA provides sensitivity to detect the affinity ligand contamination to less than 1 ng/mL. The assay is designed to minimize interference and to provide accurate quantitation even in the presence of the protein of interest. The HSA Ligand Leakage ELISA kit can be used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product.

For use with equipment: Microplate Reader