Thermo Scientific™ DNase I Solution (1 unit/µL), RNase-free

Thermo Scientific™ DNase I Solution (1 unit/µL), RNase-free

Thermo Scientific DNase I removes unwanted DNA from cell lysates to improve protein extraction efficiency.

Deoxyribonuclease I (DNase I) is a single, glycosylated polypeptide that degrades unwanted single- and double-stranded DNA. The enzyme works by cleaving DNA into 5' phosphodinucleotide and small oligonucleotide fragments. DNase I is commonly added to cell lysis reagents to remove the viscosity caused by the DNA content in bacterial cell lysates or to remove the DNA templates from RNAs produced by in vitro transcription. RNase-free DNase is useful for any application requiring the digestion of DNA in which it is crucial to avoid damage to RNA.

Features

• Degrades and removes unwanted DNA from samples
• Cleaves both single-stranded and double-stranded DNA
• Compatible with Thermo Scientific Pierce Cell Lysis Reagents
• Reduces viscosity of bacterial lysates (protein extracts) to facilitate pipetting

Contents

• 1 mL of 10X Reaction Buffer 100 mM Tris-HCl (pH 7.5), 25 mM MgCl2, 1 mM CaCl2, 50 mM EDTA