Thermo Scientific™ DyLight™ Microscale Antibody Labeling Kits, DyLight™ 594

Thermo Scientific™ DyLight™ Microscale Antibody Labeling Kits, DyLight™ 594

Invitrogen Dylight Microscale Antibody Labeling kits provide everything needed to label 100 µg of antibody or protein with amine-reactive Dylight dye and subsequent free dye removal. Choose from a variety of Dylight fluorophores across the spectrum to covalently label your antibody or protein. Labeled antibodies are typically ready to use in 90 minutes with minimal hands-on time. The kit contains all necessary components to perform five separate labeling reactions using 100 µg of IgG or similar quantities of other proteins. Labeled antibody or protein can be used for a variety of applications including immunofluorescence microscopy, flow cytometry, IHC, western blotting, or ELISA.

Features

• High performance—high fluorescence intensity across a broad pH range
• Multiple colors—10 different dyes across the visible spectrum and extending into the infrared
• Specificity—NHS ester-activated dye labels proteins and other molecules at primary amines (-NH2)
• Optimized procedure—following the standard protocol results in antibodies with excellent dye:protein ratios and recovery rates for optimum activity and fluorescence labeling

Contents

• Contains DyLight Fluor NHS Ester, 5 x 15 μg vials; Borate Buffer (0.67 M), 1 mL; Purification Resin, 5 mL; Microcentrifuge Spin Columns, 5 columns; and Microcentrifuge Collection Tubes, 10 Tubes