Thermo Scientific™ EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride), 25 g
On demandThermo Scientific™ EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride), 25 g
Thermo Scientific Pierce EDC is a carboxyl- and amine-reactive zero-length crosslinker. EDC reacts with a carboxyl group first and forms an amine-reactive O-acylisourea intermediate that quickly reacts with an amino group to form an amide bond with release of an isourea by-product. The intermediate is unstable in aqueous solutions and so two-step conjugation procedures rely on N-hydroxysuccinimide (NHS) for stabilization. Failure to react with an amine will result in hydrolysis of the intermediate, regeneration of the carboxyl, and release of an N-substituted urea. A side reaction is the formation of an N-acylurea, which is usually restricted to carboxyls located in hydrophobic regions of proteins.
Thermo Scientific No-Weigh products are specialty reagents provided in a pre-aliquoted format. The pre-weighed packaging prevents the loss of reagent reactivity and contamination over time by eliminating the repetitive opening and closing of the vial. The format enables use of a fresh vial of reagent each time, eliminating the hassle of weighing small amounts of reagents and reducing concerns over reagent stability.
Features
- Reactive group: carbodiimide
- Reaction target: activates carboxyl groups to conjugate to amino groups (primary amines)
- Several conjugation strategies—react EDC alone with target groups or include NHS or Sulfo-NHS to increase reaction efficiency or to stabilize active intermediate for later reaction to amines
- Neutral linkage—forms neutral amide bonds between carboxyls and amines
- Water-soluble reagent—add directly to reactions in aqueous, physiological buffers
- Soluble reaction byproducts—easily removed by washing with water or dilute acid
- High-purity, crystalline reagent—use to create high-quality, activated derivatives
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