Thermo Scientific™ EZ-Link™ BMCC-Biotin

Thermo Scientific™ EZ-Link™ BMCC-Biotin

Thermo Scientific EZ-Link BMCC-Biotin is a maleimide-activated, sulfhydryl-reactive biotinylation reagent with an extended spacer arm that contains a stabilizing cyclohexane group.

BMCC-Biotin is a maleimido-biotin compound for labeling protein cysteines and other molecules that contain sulfhydryl groups. This reagent specifically reacts with reduced thiols (-SH) in near-neutral buffers to form permanent (irreversible) thioether bonds. The unique feature of BMCC-Biotin is its spacer arm cyclohexane ring; this has a stabilizing effect that minimizes hydrolysis and degradation of the maleimide group until it has opportunity to conjugate with target thiols.

Features

• Protein labeling—biotinylate antibodies or other proteins for use in protein methods
• Membrane-permeable—can be used to label inside cells (intracellular)
• Thiol-reactive—reacts with sulfhydryls (-SH), such as the side-chain of cysteine (C)
• Maleimide-activated—perform reactions at pH 6.5 to 7.5 in buffers such as PBS
• Irreversible—forms permanent thioether bonds; spacer arm cannot be cleaved
• Solubility—must be dissolved in DMSO or DMF before further dilution in aqueous buffers
• Medium length—spacer arm (total length added to target) is 32.6 angstroms; contains cyclohexane ring, which stabilizes adjacent maleimide