Thermo Scientific™ EZ-Link™ DBCO Protein Labeling Kit

Thermo Scientific™ EZ-Link™ DBCO Protein Labeling Kit

The EZ-Link DBCO Protein Labeling Kit provides everything needed to covalently label proteins with a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry applications. This kit contains the necessary materials to perform five labeling reactions and subsequent purification for removal of excess DBCO reagent. The TFP-PEG4-DBCO provided in this kit readily reacts with the primary amines found on proteins to form a covalently attached DBCO group that can be further reacted with an azide-labeled coupling partner (supplied separately) to create diverse bioconjugates.

Features

• Versatile—label proteins (20–150 kDa) with volumes of 0.5–5 mL
• Chemoselective—DBCO reacts selectively with azides
• Heterobifunctional—reactive towards amine and azides
• Stable—TFP esters are more hydrolytically stable than NHS esters enabling less reagent to be used
• Convenient—labeling and purification can be completed in as little as 90 minutes with 30 min hands-on time

Contents

• Contains 5 vials of TFP Ester-PEG4-DBCO, 5 Zeba Dye & Biotin Removal columns, anhydrous DMSO, and BupH™ Phosphate Buffered Saline pack