Thermo Scientific™ EZ-Link™ Hydrazide-PEG4-Desthiobiotin, No-Weigh™ Format

Thermo Scientific™ EZ-Link™ Hydrazide-PEG4-Desthiobiotin, No-Weigh™ Format

Thermo Scientific EZ-Link Hydrazide-PEG4-Desthiobiotin is a pegylated, carbonyl-reactive labeling reagent whose biotin-like group is elutable from streptavidin, enabling its use for cell surface glycoprotein labeling and purification.

Features

• Desthiobiotin—analog of biotin allows easy elution from streptavidin, an ideal feature for affinity purification applications
• Glycoprotein labeling—tag glycosylated proteins at sialic acid residues to enable high recovery in pull-down assays with streptavidin beads
• Cell surface labeling—tag and isolate cell surface glycoproteins; reagent does not permeate membranes of whole cells
• Aldehyde-reactive—reacts with aldehydes formed by periodate-oxidation of sugar groups
• Hydrazide-activated—perform reactions at pH 4 to 6 in buffers such as sodium acetate
• Pegylated—spacer arm contains a hydrophilic, 4-unit, polyethylene glycol (PEG) group
• Enhances solubility—pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution