Thermo Scientific™ EZ-Link™ Maleimide Activated Horseradish Peroxidase Kit

Thermo Scientific™ EZ-Link™ Maleimide Activated Horseradish Peroxidase Kit

Thermo Scientific Pierce Maleimide Activated Horseradish Peroxidase Kit is for preparation of HRP conjugates with proteins, peptides or other ligands that contain sulfhydryl groups, such as reduced cysteines. It is sufficient fo the conjugation of 5 mg of immunoglobulin (IgG). The kit contains all buffers and one 10 mL desalting column.

This product consists of horseradish peroxidase (HRP) that has been modified with Sulfo-SMCC (Part No. 22322) to attach several maleimide groups per HRP molecule while retaining the peroxidase activity. The activated HRP will covalently attach to proteins or other molecule containing sulfhydryl groups (e.g., cysteines). HRP-conjugates of antibodies, proteins, peptides and other thiol-containing reporter probes are easily made using this method. The complete kit includes the activated HRP as well as two types of reagents for preparing sulfhydryl-ready antibodies (IgG) or proteins for conjugation.

Features

• Activated HRP – horseradish peroxidase (HRP) modified with maleimide groups for conjugation to sulfhydryl molecules
• Sulfhydryl-reactive – maleimide groups conjugate to reduced thiols (-SH), as in the side-chain of cysteine residues
• High activity HRP – enzyme activity is greater than 240 units/mg; lyophilized, activated enzyme is stable for at least 12 months at 4°C
• Complete kit – includes the activated HRP as well as two types of reagents for sulfhydryl-ready antibodies (IgG) or proteins for conjugation

Contents

Sufficient For: 1 enzyme-labeling reaction with up to 5 mg of antibody (IgG)

• Maleimide Activated Horseradish Peroxidase, 5 mg
• Conjugation Buffer (10X), 20 mL
• PBS Pack (makes 500mL), 1 pack
• 2-Mercaptoethylamine-HCl, 6 mg
• SATA, 2 mg
• Dimethylformamide, 1 mL
• Hydroxylamine-HCl, 5 mg
• Polyacrylamide Desalting Column, 10 mL, 1 column