Thermo Scientific™ EZ-Link™ Maleimide-PEG Solid-Phase Biotinylation Kit - Mini-Spin Columns

Thermo Scientific™ EZ-Link™ Maleimide-PEG Solid-Phase Biotinylation Kit - Mini-Spin Columns

The Thermo Scientific EZ-Link Maleimide-PEG Solid-Phase Biotinylation Kit provides an on-column approach to sulfhydryl-targeted antibody biotinylation that provides greater control over the labeling reaction and clean-up process.

This innovative antibody-labeling system uses nickel-chelated agarose to temporarily immobilize antibody molecules via their histidine-rich Fc regions. Once held in place on the resin, the antibody can be gently reduced at the hinge-region to produce free sulfhydryls that are then biotinylated with the Maleimide-PEG2-Biotin reagent. Excess labeling reagent and byproducts are then washed away before recovering the labeled and purified antibody from the resin using a mild imidazole solution. No gel filtration or dialysis is needed.

Features

• Ideal for small-scale reactions—0.1 to 1 mg antibody
• Fast labeling and purification—the entire procedure takes only about two hours
• Easy removal of spent and excess labeling reagent—simply wash away the reaction byproducts—no need for dialysis or gel filtration
• No dilution effects—solid-phase method allows initially dilute antibodies to be recovered in a smaller volume after labeling
• Optimized protocols—specific protocols for antibody ensure gentle disulfide reduction and appropriate level of labeling to minimize the possibility of inactivation
• High-performance biotin reagent—Malemide-PEG2-Biotin uses efficient, sulfhydryl-specific maleimide chemistry and includes hydrophilic polyethylene glycol (PEG) spacer that stabilizes long-term solubility of labeled antibodies
• Eight biotin-labeling experiments per kit—Thermo Scientific No-Weigh Packaging ensures that the biotin reagent is fully active for eight separate experiments

Contents

Sufficient For: 8 labeling reactions, each with 0.1 to 1 mg of antibody

• Ni-NTA Spin Columns, 0.2 mL, 8 columns
• TBS pack (makes 500mL), 1 pack
• Maleimide-PEG2-Biotin, 8 x 2 mg
• Imidazole Stock Solution (4M), 5 mL
• Collection Tubes (2mL), 30 tubes