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Thermo Scientific™ EZ-Link™ NHS-LC-LC-Biotin

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Order number : 21343
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Thermo Scientific™ EZ-Link™ NHS-LC-LC-Biotin


Thermo Scientific EZ-Link NHS-LC-LC-Biotin is a doubly long-chain, NHS-ester activated biotinylation reagent for labeling primary amines (e.g., protein lysines), whose membrane permeability enables it to be used for intracellular labeling.

NHS-LC-LC-Biotin is succinimidyl-6-(biotinamido)-6-hexanamido hexanoate. It is the longest of three EZ-Link NHS-Biotin Reagents that enable simple and efficient biotinylation of antibodies, proteins and any other primary amine-containing biomolecules in solution. Differing only in their spacer arm lengths, the three NHS-ester reagents offer researchers the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor. Because they are uncharged and contain simple alkyl-chain spacer arms, these biotin compounds are membrane-permeable and useful for intracellular labeling.


Features

  • Protein labeling—biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins
  • Membrane-permeable—can be used to label inside cells (intracellular)
  • Amine-reactive—reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the amino-termini of polypeptides
  • Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
  • Solubility—must be dissolved in DMSO or DMF before further dilution in aqueous buffers
  • Long reach—spacer arm (total length added to target) is 30.5 angstroms; it consists of the biotin valeric acid group extended by a 13-atom chain


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