Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Desthiobiotin, No-Weigh™ Format

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Desthiobiotin, No-Weigh™ Format

Thermo Scientific EZ-Link Sulfo-NHS-LC-Desthiobiotin is a long-chain, amine-reactive labeling reagent whose biotin-like group is elutable from streptavidin, making it ideal for purified or cell surface protein labeling and purification.

Sulfo-NHS-LC-Desthiobiotin is a variant of biotin that is activated as a sulfo-NHS ester with a long-chain (LC) spacer arm to covalently label primary amines (-NH2) of proteins or other molecules with desthiobiotin groups. The desthiobiotin tag binds to streptavidin and other biotin-binding proteins with high specificity yet readily elutes with mild conditions (i.e., by competitive displacement with regular, free biotin). As such, this reagent is a useful alternative to Sulfo-NHS-LC-Biotin (Part No. 21335) for avidin-biotin techniques in which nondenaturing elution of the labeled proteins is desired. The negatively charged sulfo-NHS group prevents this reagent from permeating cell membranes; therefore, Sulfo-NHS-LC-Desthiobiotin can be used to confine labeling to cell surface proteins of intact cells. The No-Weigh Format (5 x 1 mg resealable vials) eliminates the difficulties associated with weighing small quantities of reagent and maximizes protection of unused reagent from hydrolysis.

Features

• Desthiobiotin—analog of biotin allows easy elution from streptavidin, an ideal feature for affinity purification applications
• Protein labeling—tag antibodies or proteins in purified or mixed samples to enable high recovery in pull-down assays with streptavidin beads
• Cell surface labeling—modifies only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
• Amine-reactive—reacts with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides
• Soluble—charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds