Thermo Scientific™ EZ-Link™ TFP Ester-PEG4-DBCO, 25 mg

Thermo Scientific™ EZ-Link™ TFP Ester-PEG4-DBCO, 25 mg

EZ-Link TFP Ester-PEG4-DBCO is an amine-reactive hetero-bifunctional crosslinker that contains a dibenzylcyclooctyne (DBCO) group for copper-free click-chemistry applications. This crosslinker can be used to label a variety of amine-containing molecules such as proteins, biomolecules, or small molecules. Once a protein or (bio)molecule is DBCO-labeled, it can undergo a copper-free azide-alkyne cyclo-addition reaction to form a stable triazole linkage with an azide-labeled coupling partner.

EZ-Link TFP Ester-PEG4-DBCO can be used to create diverse conjugates by means of the bioorthogonal coupling between an alkyne and azide (supplied separately). The coupling or crosslinking procedure is typically done in two steps. First, a protein- or amine-containing molecule is labeled with a DBCO group and excess crosslinker is removed. Second, an azide-containing moiety (prepared independently) is introduced into the same system as the DBCO moiety and labeling occurs under biological conditions without the introduction of any additional reagents such as a copper catalyst.

Features

• Bioorthogonal—DBCO and azides react selectively to form triazole linkages
• Heterobifunctional—reactive towards amine and azide
• Soluble—reagent readily dissolves in water-miscible solvents for dilution into aqueous reaction mixtures
• Stable—TFP esters are more hydrolytically stable than NHS esters