Thermo Scientific™ GeneBLAzer™ EDG3-Gα15 HEK 293T DA Assay Kit

Thermo Scientific™ GeneBLAzer™ EDG3-Gα15 HEK 293T DA Assay Kit

GeneBLAzer™ EDG3-Gα15 HEK 293T DA (Division Arrested) cells and EDG3-Gα15-NFAT-bla HEK 293T cells contain the human EDG3 receptor stably integrated in the GeneBLAzer™ Gα15-NFAT-bla HEK 293T cell line. GeneBLAzer™ Gα15-NFAT-bla HEK 293T cells (#K1212) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element, and stably express the promiscuous G-protein, Gα15. Division Arrested (DA) cells are available in two configurations: an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates

Contents:

GeneBLAzer™ EDG3-Gα15 HEK 293T DA Assay Kit
Each system contains sufficient division arrested (DA) cells & substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol).
Includes:

• EDG3-Gα15 HEK 293T DA cells
• LiveBLAzer™-FRET B/G Loading Kit, 70µg
• Solution D, 1ml
• 2 protocols
• Certificate of Analysis

GeneBLAzer™ EDG3-Gα15 HEK 293T DA cells
Each system contains sufficient division arrested (DA) cells to assay 10 x 384-well plates (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:

• EDG3-Gα15 HEK 293T DA cells (K1320A)
• 1 protocol
• Certificate of Analysis

GeneBLAzer™ EDG3-Gα15-NFAT-bla HEK 293T cells
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:

• EDG3-Gα15-NFAT-bla HEK 293T cells (K1234)
• 1 protocol
• Certificate of Analysis