Thermo Scientific™ GeneBLAzer™ HCRTR2-Gα15 CHO-K1 DA Assay Kit

Thermo Scientific™ GeneBLAzer™ HCRTR2-Gα15 CHO-K1 DA Assay Kit

GeneBLAzer™ HCRTR2-Gα15 CHO-K1 DA(Division Arrested) cells and HCRTR2-Gα15-NFAT-bla CHO-K1 cells contain the human Hypocretin 2 receptor (HCRTR2) stably integrated into the GeneBLAzer™ Ga15-NFAT-bla CHO-K1 cell line. Gα15-NFAT-bla CHO-K1 cells (#K1213) contain a beta-lactamase reporter gene under control of the nuclear factor of activated T-cells Response Element (NFAT), as well as the promiscuous G-protein, Ga15. Division Arrested (DA) cells are available in two configurations: an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both HCRTR2-Gα15 CHO-K1 DA cells and HCRTR2-Gα15-NFAT-bla CHO-K1 cells are functionally validated for Z’-factor and EC50 concentrations of Orexin B (Figure 1). In addition, HCRTR2-Gα15-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available

Contents

GeneBLAzer™ HCRTR2-Gα15 CHO-K1 DA Assay Kit
Each system contains sufficient division arrested (DA) cells & substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol).
Includes:

• HCRTR2-Gα15 CHO-K1 DA cells
• LiveBLAzer™-FRET B/G Loading Kit, 70µg
• Solution D, 1ml
• 2 protocols
• Certificate of Analysis

GeneBLAzer™ HCRTR2-Gα15 CHO-K1 DA cells
Each system contains sufficient division arrested (DA) cells to assay 10 x 384-well plates (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:

• HCRTR2-Gα15 CHO-K1 DA cells
• 1 protocol
• Certificate of Analysis

GeneBLAzer™ HCRTR2-Gα15-NFAT-bla CHO-K1 cells
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:

• HCRTR2-Gα15-NFAT-bla CHO-K1 cells
• 1 protocol
• Certificate of Analysis