Thermo Scientific™ GeneBLAzer™ P2RY6 CHO-K1 DA Assay Kit

Thermo Scientific™ GeneBLAzer™ P2RY6 CHO-K1 DA Assay Kit

GeneBLAzer™ P2RY6 CHO-K1 DA (Division Arrested) cells and P2RY6-NFAT-bla CHO-K1 cells contain the human purinergic receptor P2, G protein-coupled, 6 (P2RY6) receptor stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line. CellSensor™ NFAT-bla CHO-K1 cells (Cat. #K1078) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element. Division Arrested (DA) cells are available in two configurations: an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both P2RY6 CHO-K1 DA cells and P2RY6-NFAT-bla CHO-K1 cells are functionally validated for Z’-factor and EC50 concentrations of Uridine 5’-diphosphate (UDP). In addition, P2RY6-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available

Contents

GeneBLAzer™ P2RY6 CHO-K1 DA Assay Kit
Each system contains sufficient division arrested (DA) cells & substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol).
Includes:

• P2RY6 CHO-K1 DA cells
• LiveBLAzer™-FRET B/G Loading Kit, 70µg
• Solution D, 1ml
• 2 protocols
• Certificate of Analysis

GeneBLAzer™ P2RY6 CHO-K1 DA cells
Each system contains sufficient division arrested (DA) cells to assay 10 x 384-well plates (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:

• P2RY6 CHO-K1 DA cells
• 1 protocol
• Certificate of Analysis

GeneBLAzer™ P2RY6-NFAT-bla CHO-K1 cells
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:

• P2RY6-NFAT-bla CHO-K1 cells
• 1 protocol
• Certificate of Analysis