Thermo Scientific™ In-Gel Tryptic Digestion Kit

Thermo Scientific™ In-Gel Tryptic Digestion Kit

Thermo Scientific Pierce In-Gel Tryptic Digestion Kit provides a complete set of reagents for digesting and recovering peptide fragments from approximately 150 stained protein bands excised from SDS-PAGE (polyacrylamide gel plugs).

The In-Gel Tryptic Digestion Kit provides a complete set of reagents for performing approximately 150 digestions on colloidal coomassie- or fluorescent dye-stained protein bands. The kit includes modified porcine trypsin, destaining buffers, reduction reagents, alkylation reagents and digestion buffers along with detailed and simple instructions. Each component and step has been optimized and balanced to produce complete, accurate and clean digests using a variety of conditions for dependable proteomics analysis of peptide profiles by mass spectrometry (MS).

Features

• Convenient—includes all necessary reagents for destaining coomassie- or fluorescent dye-stained proteins, reduction and alkylation of cystines, and tryptic digestion
• Robust—the procedure and reagents produce reliable digestions and data generation using a wide range of conditions without optimization
• Accurate—contains highly purified and modified MS-grade trypsin that shows no chymotryptic activity and minimal autolytic activity