Thermo Scientific™ Lysozyme

Thermo Scientific™ Lysozyme

Thermo Scientific Lysozyme is an enzyme characterized by the ability to break down the bacterial cell wall to improve protein or nucleic acid extraction efficiency. This product is a lyophilized powder for large-volume applications.

Lysozyme is an enzyme used to break down bacterial cell walls to improve protein or nucleic acid extraction efficiency. Lysozymes (muramidases) are a family of enzymes with antimicrobial activity characterized by the ability to damage the cell wall of bacteria. The enzyme acts by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycans and between the N-acetyl-D-glucosamine residues in chitodextrins. Although hen egg white lysozyme is most effective for the lysis of gram-positive bacteria, it also facilitates the lysis of gram-negative bacteria such as Salmonella and Shigella. The lysis of E. coli is especially improved by the addition of both lysozyme and a nucleases such as DNase I.

Features

• Bacterial cell wall lytic enzyme that improves inclusion body protein purification
• Compatible with Thermo Scientific Pierce Cell Lysis Reagents
• White, lyophilized powder
• Approx. 20,000 units/mg dry weight
• Must be readily soluble at 5 mg/mL in 0.1M KH2PO4 to give a clear, colorless solution.