Thermo Scientific™ NAb™ Protein G Spin Columns, 5 mL

Thermo Scientific™ NAb™ Protein G Spin Columns, 5 mL

The Thermo Scientific NAb Protein G Spin Columns are convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types. Each column containing 5 mL of the immobilized protein resin enables quick purification of 5-65 mg of IgG from 2-10 mL of serum or other sample. The actual amount of IgG purified varies depending upon the sample type and the specific spin column used.

Pierce Protein G Agarose is a premium-quality affinity resin for antibody purification. It consists of recombinant Protein G that has been covalently immobilized onto high-quality crosslinked 6% beaded agarose (CL-6B). This particular variety of the affinity resin provides the most versatile combination of chromatographic features for high yield and high-purity purification of whole IgG from mammalian serum samples. The agarose beads have physical and chemical properties suitable for many affinity purification systems.

Features

• Protein G – immobilized Protein G is ideal for polyclonal IgG purification from mouse, human, cow, goat and sheep serum, including human IgG3 and mouse IgG1 isotypes
• Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
• Inert and stable – superior manufacturing method immobilizes Protein G by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
• Standard capacity – Pierce Protein G Agarose has a normal load of immobilized Protein G, providing a binding capacity of 11 to 15 mg human IgG/mL resin