Thermo Scientific™ NAb™ Protein L Spin Columns, 0.2 mL

Thermo Scientific™ NAb™ Protein L Spin Columns, 0.2 mL

The Thermo Scientific NAb Protein L Spin Columns are convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types. Each column containing 0.2 mL of the immobilized protein resin enables quick purification of 100-1,000 µg of IgG from 25-500µL of serum or other sample. The actual amount of IgG purified varies depending upon the sample type and the specific spin column used.

Pierce Protein L Agarose consists of recombinant Protein L that has been covalently immobilized onto high-quality crosslinked 6% beaded agarose (CL-6B). This particular variety of the resin provides the most versatile combination of chromatographic features for high yield and high purity purification of target immunoglobulins. The agarose beads have physical and chemical properties suitable for many affinity purification systems.

Features

• Protein L – immobilized Protein L is ideal for selective purification of human and mouse antibodies that have kappa light chains
• Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
• Inert and stable – superior manufacturing method immobilizes Protein L by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
• Standard capacity – Pierce Protein L Agarose has a normal load of immobilized Protein L, providing a binding capacity of 5 to 10 mg human IgG/mL resin