Thermo Scientific™ PageRuler™ Unstained Broad Range Protein Ladder

Thermo Scientific™ PageRuler™ Unstained Broad Range Protein Ladder

Thermo Scientific PageRuler Unstained Broad Range Protein Ladder is a mixture of 11 proteins (5 to 250 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

Features

• Reference bands—the 100, 50, and 20 kDa bands are more intense for easy orientation
• Tagged— each protein in the contains an integral Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II sequence

Contents

• Ttwo vials of 250 µL each
• Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, 0.01% bromophenol blue and 33% glycerol