Thermo Scientific™ Pierce™ Alkaline Phosphatase, 20 mg

Thermo Scientific™ Pierce™ Alkaline Phosphatase, 20 mg

This purified calf intestinal alkaline phosphatase (CIP) is supplied in Tris buffer and 50% glycerol for use in protein research methods. The main applications for alkaline phosphatase in molecular biology and protein research are to remove 5'-phosphate groups from DNA or as a reporter system for immunoassays such as ELISA. For these latter methods, the enzyme is usually conjugated to specific primary or secondary antibodies and its activity is detected with a color-forming (or light-generating) substrate.

Features

• Purified form—ready to dilute and conjugate without prior dialysis
• Concentrated—approximately 20 mg/mL (lot-specific value reported)
• High specific activity—typically greater than 1600 units/mg (lot-specific value reported)
• Tris buffered solution—supplied in 5 mM Tris, 5 mM magnesium chloride and 0.1 mM zinc chloride