Thermo Scientific™ Pierce™ Boronic Acid Resin, 10 mL

Thermo Scientific™ Pierce™ Boronic Acid Resin, 10 mL

Thermo Scientific Pierce Boronic Acid Resin enables ribonucleotide and oligonucleotide RNA isolation.

The immobilized boronic acid is an easy-to-use polyacrylamide affinity support for purification of ribonucleotides and other small molecular weight compounds that contain cis-diol groups. The ligand (m-aminophenyl-boronic acid) binds to the cis-diol groups on the sugar portion of nucleotides, forming a reversible five-member ring complex. After washing away non-bound molecules from the sample, the complex can be dissociated and the nucleotide eluted by lowering the pH or by addition of sorbitol.

Features

• Ligand: boronic acid
• Support: beaded polyacrylamide (1800 MW exclusion limit)
• Spacer: m-aminophenyl group
• Loading: 100 µMol boronate per milliliter of settled resin
• Capacity: at least 99% binding and recovery of 110 µMol AMP per milliliter of settled resin
• Format: 50% resin slurry in 0.05% sodium azide