Thermo Scientific™ Pierce™ ECL Western Blotting Substrate, 250 mL

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate, 250 mL

Thermo Scientific Pierce ECL Western Blotting Substrate is a value-priced, entry-level horseradish peroxidase (HRP) substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Features

• Sensitivity: low picogram
• Stability: 1-hr working solution stability, 1-year kit stability at 4°C
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: 1 to 2 hours
• Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–10 µg/mL)
• Recommended secondary antibody concentration: 1:1,000–1:15,000 dilution (0.07–1.0 µg/mL)