Thermo Scientific™ Pierce™ Fluorescent Protease Assay Kit
On demandThermo Scientific™ Pierce™ Fluorescent Protease Assay Kit
The Thermo Scientific Pierce Fluorescent Protease Assay Kit measures total protease activity in samples, providing a means to assess the progress of protease isolation procedures or quantify protease contamination in biological samples.
This kit includes fluorescein-labeled casein as a substrate for assessing protease activity in a sample by either fluorescence resonance energy transfer (FRET) with a standard fluorometer or fluorescence polarization (FP) with capable instrumentation. FTC-Casein is native casein that has been labeled using a large molar excess of fluorescein isothiocyanate (FITC). Fluorescence properties of this heavily-labeled, intact protein substrate change dramatically upon digestion by proteases, resulting in a measurable indication of proteolysis.
For use with equipment: Microplate Reader
Features
- Casein substrate—measure activity of any protease that cleaves casein into peptide fragments
- Trypsin standard—quantify protease activity relative to trypsin, a universally accepted reference
- Sensitive—1000 times more sensitive than assays that use unmodified forms of casein
- Simple and fast—test tube and microplate protocols are completed in less than one hour
- Homogenous—addition steps only; no separation, transfer or stop steps required
- Customizable—easily adapt time, temperature and pH to optimize sensitivity for proteases of interest
Contents
- FTC-Casein, lyophilized, 2.5 mg
- TPCK Trypsin Standard, 50 mg
- TBS Buffer Pack (makes 500 mL), 1 pack
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