Thermo Scientific™ Pierce™ Gaussia-Firefly Luciferase Dual Assay Kit, 1000 Reactions

Thermo Scientific™ Pierce™ Gaussia-Firefly Luciferase Dual Assay Kit, 1000 Reactions

The Thermo Scientific Pierce Gaussia-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Gaussia and Red Firefly luciferase activity in mammalian whole cell lysates.

The Pierce Gaussia-Firefly Luciferase Dual Assay Kit is a highly sensitive assay that allows for the simultaneous detection of Gaussia and Red Firefly luciferase activity. Gaussia luciferase acts as an experimental reporter with constitutively active red firefly as a normalization control. As shown in the animation videos below, this reporter-and-control combination enables simultaneous monitoring of experimental reporter and control luciferase activities in a single-read assay without the need for two-step addition of substrate reagents or quenching. The assay working solution contains substrates for both luciferases, and the reactions occur simultaneously with flash-type kinetics. The resulting luminescent signals are spectrally resolvable using filters. In a single sample, researchers can assay transcriptional activity of regulatory elements, signal transduction pathways, and effects of activators or inhibitors.

For use with: Luminometer (Microplate)

Features

• Simultaneous—concurrent, filter-based, wavelength-separated detection of two luciferase activities
• Sensitive—measure bright blue Gaussia and red firefly luciferase activities in the same sample
• Fast—no quenching step required, unlike in traditional sequential dual assays
• Multiplex—capable of quantitating two cellular activities in the same sample(s)

Contents

• Gaussia-Firefly Dual Assay Buffer, 5 mL, store at 4°C
• Coelenterazine (1X), 0.5 mL, store at -80°C
• D-Luciferin, Lyophilized, 3 mg, store at 4°C
• 2X Cell Lysis Buffer, 6 mL, store at room temperature